A scientist makes primers specific to a particular STR fragment in elephants. A genomic clone of about 3.0 kb was isolated and sequenced. Computer analysis of the sequence indicated that, similar to the androgen receptor gene, no TATA or CAAT boxes are present in the promoter region of the GR gene. 11. Chloroplast genome sequences are essential to build transgene cassettes because they provide both flanking and regulatory sequences. These primers are then used to amplify the STR fragment from 10 different elephants. 40ft. High throughput sequencing techniques work by generating large libraries of short (50-200 bases, usually) DNA fragments and determining their sequences in parallel. (57). These primers are then used to amplify the STR fragment from 10 different elephants. Answer: very STR has multiple alleles, or variants, each defined by the number of repeat units present or by the length of the sequence. The density of RAD tags in a genome depends on the restriction enzyme used during the isolation process. of movement is enough to run almost a complete circle around a medium creature, a size of creature most players are expected to face most of the time. 30ft. Explain your answer. 10. The 5′ flanking region of the human GR was first isolated and characterized by Zong et al. Notice that with each stack, my infantry outnumber both my Cavalry and My artillery. Explain your answer. for a forty stack, I do 20 Infantry, 8 Cavalry, and 12 Artillery. This is really a fascinating question of tactics and the correct answer depends on what armies in what time period under what leadership are facing one another! Would you expect the fragment to be the same size in all the elephants? Why are flanking sequences important for amplifying STR fragments? We confirm the important role of flanking sequences using binding assays. Transgene cassettes that are inserted into bacterial plasmids are called chloroplast vectors and they are bombarded into plant cells using gold particles and a gene gun (Fig. This is actually an important point. Highly divergent N‐ and C‐terminal flanking sequences are important for the function of sHsps, as the isolated α‐crystallin domains investigated so far show no chaperone activity (22 – 24). Of course you didn’t really expect a simple answer did you? That is why you need a good army composition. The structures show that the amino acids N-terminally flanking the basic cluster make specific contacts with the receptor that are distinct from the interactions between bipartite NLSs and Impα. 11. The use of the flanking DNA sequences around each restriction site is an important aspect of RAD tags. ... 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